|
|
Root number
|
2102 |
|
Semester
|
HS2025 |
|
Type of course
|
Lecture |
|
Allocation to subject
|
Biochemistry |
|
Type of exam
|
Written exam |
| Title |
Methods in Biochemistry I with Exercises |
| Description |
Protein(bio)chemie (7 Wochen)
1. Einführung: Zellen, Proteine, Aminosäuren
2. Proteinreinigung in mehreren Schritten (Zentrifugation bis Chromatographie), mit Spezialfall Membranprotein
3. Proteindetektion (Elektrophorese, Mengenbestimmung, Aktivität)
4. Proteinmodifikation, -spaltung & -analytik (Sequenzierung, Aminosäureanalyse, Massenspektrometrie)
5. Arbeiten mit Membranproteinen (Detergenzien, Liposomen & Nanodiscs)
DNA-Methoden (2 Wochen)
1. Geschichte der DNA Sequenzierung
2. PCR & DNA Fingerprinting
3. Klonieren von DNA
4. Mutagenese
5. Gen Knockout
6. Genome editing
7. cDNA Bibliotheken
Cell culture and virological methods (2 weeks) - This part will be taught in English.
1. Basic cell culture techniques: Maintaining cells in culture, passage, counting, viability, and storage
2. Basic cell methods: Cell fractionation, flow cytometry, and transfection
3. Introduction to virology. Virus quantification/infectivity
4. Detection of viruses infecting cells: Immunofluorescence microscopy
5. Detection and quantification of viruses: RNA/DNA extraction, qPCR, and RT-qPCR. Immunoprecipitation
Radiochemie und Strahlenschutz (3 Wochen):
Die Vorlesung behandelt nach einer Einführung in die Radiochemie, nukleare Methoden, die in der biochemischen Forschung Verwendung finden. Ebenfalls werden die Wechselwirkung radioaktiver Strahlung mit Materie und die daraus abgeleiteten Strahlenschutzgrundsätze und Arbeitsweisen diskutiert.
Übungen zur Vorbereitung der Prüfung. |
|
ILIAS-Link (Learning resource for course)
|
Registrations are transmitted from CTS to ILIAS (no admission in ILIAS possible).
ILIAS
|
|
Link to another web site
|
|
| Lecturers |
Prof. Dr.
Norbert Polacek, Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) ✉
|
|
PD Dr.
Carlos Ros Bascunana, Departement of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP), Chemistry & Biochemistry ✉
|
|
Prof. Dr.
Andreas Türler, Departement of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP), Chemistry & Biochemistry ✉
|
|
Prof. Dr.
Christoph von Ballmoos, Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) ✉
|
|
ECTS
|
3 |
|
Recognition as optional course possible
|
Yes |
|
Grading
|
1 to 6 |
| |
| Dates |
Tuesday 15:15-16:00 Weekly
|
|
Tuesday 13:15-15:00 Weekly
|
|
Tuesday 20/1/2026 14:00-16:00
|
| |
| Rooms |
Hörsaal U113, Chemie, Biochemie und Pharmazie, DCBP
|
|
Studer-Auditorium 235, Geologie, Bal 3
|
| |
| Students please consult the detailed view for complete information on dates, rooms and planned podcasts. |
User:
Öffentlicher Bereich
Support